The reliability of the bactec mgit 960 system, an automated version of the. Tubes fit into standard procedures for decontamination and waste disposal. Comparison of the mgit 960, bactec 460 tb and solid media for. Bbl mgit mycobacteria growth indicator tube 7 ml with. Comparison of the recovery of mycobacterium bovis isolates. The objective of the study was to compare the manual mycobacteria growth indicator tube mgit method and the bactec mgit 960 system to the bactec 460 method for susceptibility testing of mycobacterium tuberculosis. They provide optimal environments for the recovery of a wide range of microorganisms, including bacteria, fungi and mycobacteria. Instrument number r, 14, 1, 4 the user configured instrument number that ranges from 1 to 99. The bd bactec mgit automated mycobacterial detection system is a fully automated solution for mycobacterial liquid culture and susceptibility testing. Original article the manual mgit system for the detection of. The time to detection for the different species of mycobacteria isolated was less with bactec mgit 960 as compared to bactec 460. This test is now automated for continuous monitoring in the bactec mgit 960 becton. Bd leads in health care safety including medication safety, health care worker safety. Becton dickinson mgit 960 bactec automated mycobacterial.
Comparison of bactec 460 and mgit 960 systems for the culture. Manufacturer specifications bactec mgit 960, becton dickinson. We compared the bactec mgit 960 to the bactec 460 tb system and the conventional lj medium. Dickinson but the older manual system requires tubes to be examined.
Deteksi mikobakterium menggunakan manual mgit dan bactectm mgittm 960 system didik agus santoso,dr dr. Bactec mgit 960 mycobacterial standard operating procedure rapid molecular tests such as the manual line. Currently the bactec mgit 960, bactec 9000, bd phoenix, bactec fx and the bactec fx40 can be connected to the epicenter system. Pdf evaluation of the bactec mgit 960 system for the. The new bd bactec mgit 960 system is the next generation. The bd bactec mgit 960 system is for highvolume mycobacteria growth, detection and susceptibility testing and is designed to meet the needs of medium and highvolume laboratories. Though requiring more technical time, the manual system is a lot more affordable for laboratories with a smaller budget.
Delamanid susceptibility testing of mycobacterium tuberculosis using the resazurin microtitre assay and. Mycobacteriology laboratory manual i this document was developed to ensure high quality results and comparability of data from a network of international tuberculosis. Mar 25, 2019 the epicenter system provides an easy to use graphical interface that allows the laboratory user to consolidate and manage their clinical testing data from multiple bd diagnostic instruments. The bbl mgit mycobacteria growth indicator tube supplemented with bactec mgit growth supplement and bbl mgit panta antibiotic mixture is intended for the detection and recovery of mycobacteria using the bactec mgit 960 and bactec mgit 320 systems. Baud rate acceptable values are 1200, 2400, 4800, 9600. The bactec mgit 960 system is designed for the rapid detection of mycobacteria in all types of clinical specimens except blood and urine. Mycobacterium tb susceptibility testing public health. With these technical advantages, we went on to assess the performance of the system for the detection of mycobacteria. Configurable options lis enable acceptable values are enable or disable. Potential for erroneous results indicating resistance when. Procedure compatibility bd bactec mgit tubes fit into standard procedures for decontamination naclnaoh and waste disposal. Bd bbl mgit panta antibiotic mixture is intended for the detection and recovery of mycobacteria using the bd bactec mgit 960 and bd bactec mgit 320 systems.
Instrument location r, 14, 1, 5 the specimens location within the instrument. Its performance was compared to those of the radiometric bactec 460 instrument and eggbased lowensteinjensen. Comparison of bactec mgit 960 and bactec 460 for culture of. We have compared the bactec 460 system with the bactec mgit 960 system for culture of mycobacteria from 1800 routine clinical specimens.
Bactec mgit 960 system principle, manual and brochure the bactec mgit 960 mycobacterial detection system is the worlds first automated system for highvolume mycobacteria growth, detection and susceptibility testing from becton dickinson bd. A total of 506 bovine lymph node samples were collected from abattoirs in the united states and mexico between november 2003 and september 2004. The first culture of mtbc isolated from a patient is routinely tested for susceptibility to each of the four first line anti tuberculosis drugs isoniazid inh, rifampin rif, ethambutol emb and pyrazinamide pza. Comparison of the bactec 460tb system and the bactec mgit. Class 2 device recall bd bactectm mgit tm 960 system. Dec 12, 20 deteksi mikobakterium menggunakan manual mgit dan bactectm mgittm 960 system 1. Mgit early positive cultures znbap negative 46 11 solid culture. Using this concentration, the sensitivity of both mgit methods was 100%, but the specificities were 72 and 69% for the mgit 960 and the manual mgit, respectively. No other blood culture system has currently demonstrated a shorter protocol or a higher recovery rate than the bd bactec 9000.
At this time no other complaints have been reported for this product lot. Fluorescence can be recorded by automated instruments such as becton dickinsons bactec mgit 960 system, or manually using the bd bactec micromgit fluorescence reader or a woods lamp or other longwave uv light source. Susceptibility testing with the manual mycobacteria growth. For each strain, a bacterial suspension m was prepared from mgit subcultures according to the manufacturers instructions bactec mgit 960 system user s manual. Bactec mgit 960 system principle, manual and brochure the new generation automated system for mycobacterial culture and sensitivity using mgit tubes. The epicenter system provides an easy to use graphical interface that allows the laboratory user to consolidate and manage their clinical testing data from multiple bd diagnostic instruments. Bd is a leading medical technology company that advances human health in every corner of the world by improving medical research, diagnostics and the delivery of care. The bactec tm mgit tm system software is used in the mgit 960 and mgit 320 instruments for the rapid detection of mycobacteria from clinical specimens. The bd bactec mgit 960 system is the next generation in. The bactec mgit 960 results were compared with those obtained with the radiometric bactec 460tb system, and when there was disagreement, the method of proportion on agar plates. The bactec mgit 960 mycobacterial detection system is the worlds first automated system for highvolume mycobacteria growth, detection and susceptibility testing from becton dickinson bd. The results of susceptibility to rmp were all in agreement.
We would like to show you a description here but the site wont allow us. Bbl mgit mycobacteria growth indicator tubes and bactec mgit 960 supplement kit. For specimens suspected of containing mycobacterium haemophilum, a. Bd bactec tm mgi tm system software, catalog 445922, lot v5. Apr 11, 20 at the national veterinary services laboratories the bactec mgit 960 and 4 solid media formulations were compared with the bactec 460 tb system on 6,795 veterinary diagnostic specimens submitted for mycobacterium bovis culture. The mgit mycobacteria growth indicator tube contains 7 ml of modified middlebrook 7h9 broth base. The second method, known as the bactec 460tb radiometric susceptibility. The manual mgit system for the detection of m tuberculosis in. To use, supplement tube with mgit oadc enrichment and mgit panta antibiotic mixture, inoculate with the prepared specimen and incubate. Bd bactec mgit tubes only require the addition of the bd bactec mgit 960 supplement prior to specimen inoculation and incubation. Bd bactec bd peds plus medium culture vials enriched soybean casein digest broth with co2 are used for the culture and recovery of aerobic microorganisms mainly bacteria and fungi from paediatric and other blood specimens which are generally less than 3 ml in volume.
Bd bactec mgit automated mycobacterial detection system bd. Performance of the bactec mgit 960 compared with solid. Used becton dickinson, bactec mgit 960, microbiological. Performance of the bactec mgit 960 compared with solid media for detection of mycobacterium in bangkok, thailand article in diagnostic microbiology and infectious disease 614. General algorithm mgit 960 cultures 43 flow chart 2. It is a continuous monitoring system and hence requires less hands on for the technologist. Procedure manual for bactec mgit 960 tb system semantic scholar pdfs. Bbl mgit mycobacteria growth indicator tube 7 ml with bactec mgit 960 supplement kit english. Its performance was compared to those of the radiometric bactec 460 instrument and eggbased lowensteinjensen medium. Bd bactec mgit mycobacteria growth indicator tubes bd.
Mycobacterium tb susceptibility testing public health ontario. For information on the bactec 960 see bactec mgit 960 user s manual. The bd bbl mgit mycobacteria growth indicator tube supplemented with bd bactec mgit growth supplement and bd bbl mgit panta antibiotic mixture is intended for the detection and recovery of mycobacteria using the bd bactec mgit 960 and bd bactec mgit 320 systems. Bd bactec mgit tubes fit into standard procedures for decontamination naclnaoh and waste disposal. Apr 24, 2012 this study was carried out at the department of microbiology, armed forces institute of pathology, rawalpindi, pakistan. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Another problem when dealing with bactec mgit 960 is the frequent disagreement, albeit temporary, between the positivity reported by the system and the lack of afb in the smear. Use of the bactec mycobacteria growth indicator tube 960.
For sm, 78 and 72% of the results obtained by the mgit 960 and the manual mgit systems, respectively, agreed with the bactec 460 results. Ast set carrier see bactec mgit 960 user s manual, ast instructions. This products resides on a fisher scientific gsa or va contract. Deteksi mikobakterium menggunakan manual mgit dan bactectm. The bactec mycobacteria growth indicator tube 960 mgit 960 system was applied for recovery of mycobacteria from extrapulmonary specimens and compared with solid media lowensteinjensen and stonebrink. A total of 144 mtb clinical isolates with a strong bias toward drug resistance were examined using bactec mgit 960, sensititre mycotb, and a microarraybased molecular assay tbtest to detect substitutions in the rpob, katg, inha, ahpc, gyra, gyrb, rrs, eis, and embb genes that are associated with resistance to rifampin, isoniazid, fluoroquinolones, secondline injectable drugs and. Results are the most important aspect of any microbial detection system. In fact, in 11 cases, afb was identified on the smears a few days after the signal in bactec mgit 960 was positive. Citeseerx document details isaac councill, lee giles, pradeep teregowda.
Bd bactec mgit automated mycobacterial detection system. The instrument scans the mgit every 60 minutes for increased fluorescence. Evaluation of bactec mycobacteria growth indicator tube mgit. The purpose of this study was to find out the recovery rate of mycobacteria on mgit 960 system and to compare it with the already in use bactec 460 tb system. Usefulness of the bactec mgit 960 system for isolation of mycobacterium tuberculosis from sputa subjected to longterm storage. The following higher concentrations of s, i, and e are available for bactec mgit 960 system. This instrument is produced by becton dickinson bd. Use of bactec mgit 960 for recovery of mycobacteria from. Comparison of bactec 460 and mgit 960 systems for the culture of mycobacterium avium subsp paratuberculosis s strain and observations on the effect of inclusion of. Original article the manual mgit system for the detection. Pdf mgit procedure manual for bactec mgit 960 tb system.
Evaluation of the bactec mgit 960 system for the recovery of mycobacteria. The bd bactec mgit 320 system is designed to help smallercapacity laboratories quickly and accurately detect tb. Evaluation of automated bactec mgit 960 system for testing. Susceptibility testing for mtbc is performed using the bactec mgit 960 method, becton dickinson, md. Bactec mgit 960 system principle, manual and brochure.
Shop online for a wide selection of bd bbl mgit mycobacteria growth indicator tube. Comparison of the bactec 460tb system and the bactec mgit 960. Comparison between mgit 960 and bactec 460 tb system. We evaluated the performance of bactec mgit 960 for automated testing of the susceptibility of 3 strains of mycobacterium tuberculosis to streptomycin, isoniazid, rifampin, and ethambutol. Guides the simple 4step operating procedure, eliminating potential errors. Mycobacteriology laboratory manual world health organization. The bactec mgit 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Potential for erroneous results indicating resistance when using the bactec mgit 960 system for testing susceptibility of mycobacterium tuberculosis to pyrazinamide pamela chedore, 1, lina bertucci, 1 joyce wolfe, 2 meenu sharma, 2 and frances jamieson 1. Bactec mgit 960 fluorometric analyzer for tuberculosis, culture, differentiation and susceptibilty for antituberculosis drugs. The bactec 460 instrument tests for the presence 14co2 by removing the gas operation and maintenance manual for the bactec 460tb system, becton bactec 460 service manual. Positive tubes, identified by the bactec mgit 960 instrument, should be subcultured and an acid fast smear prepared. Another important aspect of the study is the comparison of time to detection.
Evaluation of the bactec mgit 960 system for the recovery. Manufacturer specifications bactec mgit 960, becton dickinson please note. The objective of the study was to compare the manual mycobacteria growth indicator tube mgit method and the bactec mgit 960 system to the bactec 460 method for susceptibility testing of. Bbl mgit mycobacteria growth indicator tube 7 ml with bd. A comparison of the sensititre mycotb plate, the bactec. Becton dickinson bactec mgit 960 microbiological culture analyzer for sale. A total of 9,558 specimens were investigated, comprising 3,074 body fluids, 1,878 tissues, and 2,069 urine samples, from which the recovery of mycobacteria was not yet established for mgit. Reagents offers superior detection time in a user friendly, fluorescent test format. Bd fos is provided in a lyophilised form along with a special bd bactec fos reconstituting fluid bd fos rf for use with bd bactec media to enhance the growth of fastidious organisms, such as haemophilus spp. It is specially designed to accommodate mycobacteria growth indicator tube mgit and incubate them at 37c.